Si: Intrinsic sieving [-]
TFF: Tangential flow filtration
TMP: Transmembrane pressure [bar]
UF: Ultrafiltration
VCF: Volume concentration factor [-]
VF: Virus filtration
Yield: The amount of the target product in
a pool compared to the total amount put
into the unit operation [%]
ΔP: Pressure drop [bar]
Technical References
Cheryan, M. 1986. Ultrafiltration
Handbook. Technomic Publishing Co.,
Inc., Pennsylvania.
Koros, W.J.; Ma, Y.H.; Shimidzu, T.
1996. Terminology for Membranes
and Membrane Processes (IUPAC
Recommendations 1996). Pure &
Appl. Chem. 68: 1479 – 1489.
Ng, P.; Lundblad, J.; Mitra, G. 1976.
Optimization of Solute Separation by
Diafiltration. Separation Science 2:
499 – 502.
Tkacik, G.; Michaels, S. 1991. A
Rejection Profile Test for Ultrafiltration
Membranes and Devices. Bio/Technol.
9: 941 – 946.
van Reis, R.; Gadam, S.; Frautschy,
L.N.; Orlando, S.; Goodrich, E.M.;
Saksena, S.; Kuriyel, R.; Simpson,
C.M.; Pearl, S.; Zydney, A.L. 1997.
High Performance Tangential Flow
Filtration. Biotech. Bioeng. 56:
71 – 82.
van Reis, R.; Goodrich, E.M.; Yson,
C.L.; Frautschy, L.N.; Dzegeleski, S.;
Lutz, H. 1997. Linear Scale
Ultrafiltration. Biotechnol. Bioeng. 55:
737 – 746.
van Reis, R.; Goodrich, E.M.; Yson,
C.L.; Frautschy, L.N.; Whiteley, R.;
Zydney, A.L. 1997. Constant Cwall
Ultrafiltration Process Control.
J. Membrane Sci. 130: 123 – 140.
van Reis, R.; Zydney, A.L. 1999.
Protein Ultrafiltration. In M.C. Flikinger,
S.W. Drew (eds.) Encyclopedia of
Bioprocess Technology. John Wiley
and Sons, Inc. New York.
Zeman, L.J.; Zydney, A.L. 1996.
Microfiltration and Ultrafiltration:
Principles and Applications.
Marcel Dekker, New York.
For More Information
If you would like to learn more about Tangential Flow Filtration processing, as
well as other protein purification operations, Millipore offers a variety of
information and assistance.
Training
Millipore offers several classes on TFF processing for customers. These classes
blend lecture time with hands on time in the lab to help you become proficient
in both the theory and operation of tangential flow filtration. Please visit our
website at www.millipore.com for information on the variety of training courses
available.
Expert Help
Millipore’s applications specialists have extensive knowledge of the
biotechnology and pharmaceutical fields. The AccessSM Services group works
with you to optimize the use of TFF in your facility, and get your process up and
running quickly and easily.
Discover the More in Millipore.
In every application, every step and every scale, count on Millipore to be
everywhere for you—from monoclonals to vaccines, from clinical through pilot
to full-scale manufacturing. Our technologies are used by most of the world’s
major biopharmaceutical companies. But we deliver more than advanced
separation, purification, sterilization and quality control products. With Millipore,
you get services to optimize and validate your processes, comprehensive
resources to streamline and enhance your operation, unmatched know how
forged from nearly 50 years’ experience—and solutions that integrate it all.
For higher yields, improved process economics and faster speed to market,
discover the more in Millipore.
To Place an Order or Receive Technical Assistance
For additional information call your nearest Millipore office:
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In the U.S., Canada and Puerto Rico, fax orders to 1-800-MILLIFX
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Outside of North America contact your local office. To find the office nearest you
visit www.millipore.com/offices.
Internet: www.millipore.com
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